Journal: Nature Communications

Article Title: The ABCG2 Q141K hyperuricemia and gout associated variant illuminates the physiology of human urate excretion

doi: 10.1038/s41467-020-16525-w

Figure Lengend Snippet: a , b Representative immunofluorescence micrographs of fixed cortical sections of human kidney samples (Q141) stained for ABCG2 (red), the proximal tubule marker LTL (green), the basolateral marker Na+/K+ ATPase (blue), and colocalization of ABCG2 and LTL (yellow) at 200× ( a ; scale bar 100 μM) and 400× ( b ; scale bar 50 μM). c , d Serial sections (from a , b ) stained for URAT1 (red), LTL (green), Na+/K+ ATPase (blue), and colocalization of URAT1 and LTL (yellow) at 200× ( c ) and 400× ( d ) ( a – d representative of three independently repeated experiments from n = 3 kidneys). Western blots from cortical samples ( n = 3 kidneys) and medullary samples ( n = 3 kidneys) for ABCG2 ( e ) and URAT1 ( f ). Representative immunofluorescence micrographs of fixed medullary sections of human kidney samples stained for ABCG2 (red), LTL (green), DAPI (blue), and colocalization of ABCG2 and LTL (yellow) at 200× ( g ; scale bar 100 μM) and 400× ( h ; scale bar 25 μM), or 400x without LTL ( i , j ; scale bar 25 μM) ( g – i , representative of three independently repeated experiments from n = 3 kidneys). Z projections of cultured human renal epithelial cells stained for k ABCG2 (green), Na+/K+ ATPase (red), and ZO-1 (magenta), or l URAT1 (green), Na+/K+ ATPase (red), and ZO-1 (blue)(scale bar 5 μM). Representative (similar staining was repeated on tissue from n = 3 mice) micrographs (40×; scale bar 500 μM) from fixed whole mouse kidney slices stained with ABCG2 ( m ) or URAT1 ( p ) in green, LTL (red), and colocalization (yellow). Higher magnification (400×; scale bar 20 μM) micrographs on fixed sections from mouse kidney stained for ABCG2 ( n , o ) or URAT1 ( q , r ) in red, and in o , r LTL (magenta), Na+/K+ ATPase (blue), and colocalization of ABCG2/URAT1 and LTL (pink). s Diagram of transporter localization. Source data are provided as a file.

Article Snippet: Primary antibody was added in incubation media and serum solution [rabbit anti-ABCG2 (Cell Signaling Technology 42078, 1:200), rabbit anti-URAT1 (MBL BMP064, 1:500, biotinylated LTL (Vector Labs B1325, 1:100), mouse anti-NaKATPase (Millipore 05-369, 1:200)] and incubated overnight in a humidifier chamber at room temperature.

Techniques: Immunofluorescence, Staining, Marker, Western Blot, Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: Plantaginis Semen Ameliorates Hyperuricemia Induced by Potassium Oxonate

doi: 10.3390/ijms25158548

Figure Lengend Snippet: PS significantly reversed histopathologic status, blood biochemical indexes, and mRNA expression of uric acid transporter of HUA rats. ( A ) Representative H&E staining histopathological images of potassium oxonate-induced rat kidney tissue injury treated with or without PS; the blue arrow indicates urate crystals, the yellow arrow indicates inflammatory infiltration, the red arrow indicates lobulated glomeruli, the green arrow indicates renal tubules changed into protein tubular type, and the black arrow indicates renal tubule atrophy. I. Control group. II. Model group. III. BBR group. Ⅳ. PS group. Scale bars for original images: 2000 μm, scale bars for enlarged images: 100 μm. ( B ) mRNA expression levels of Urat1 and ( C ) mRNA expression levels of Glut9 . ( D ) UA level, ( E ) XOD activity, ( F ) TNF-α level, and ( G ) IL-6 level. Values represented mean ± SD; one-way ANOVA was used for statistical analysis (** p < 0.01 and *** p < 0.001). ns: not significant.

Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibodies as follows: URAT1 (61 kD, Cat# YN3803, 1:1000, Immunoway), PPARα (52 kD, Cat# YT3835, 1:1000, Immunoway, Plano, TX, USA), and GAPDH (38 kD, Cat# YM3029, 1:1000, Immunoway, TX, USA).

Techniques: Expressing, Staining, Control, Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: Plantaginis Semen Ameliorates Hyperuricemia Induced by Potassium Oxonate

doi: 10.3390/ijms25158548

Figure Lengend Snippet: Effects of PS on URAT1 and PPARα proteins expression. ( A ) Representative immunoblots of URAT1 and URAT1 protein levels analysis. ( B ) Representative immunoblots of PPARα and PPARα protein levels analysis. n = 3. Error bars indicate SD. One-way ANOVA was used for comparing statistical significance between different groups (* p < 0.05, ** p < 0.01 and *** p < 0.001). ns: not significant.

Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibodies as follows: URAT1 (61 kD, Cat# YN3803, 1:1000, Immunoway), PPARα (52 kD, Cat# YT3835, 1:1000, Immunoway, Plano, TX, USA), and GAPDH (38 kD, Cat# YM3029, 1:1000, Immunoway, TX, USA).

Techniques: Expressing, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Plantaginis Semen Ameliorates Hyperuricemia Induced by Potassium Oxonate

doi: 10.3390/ijms25158548

Figure Lengend Snippet: Nucleotide sequences of RT-qPCR primers.

Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibodies as follows: URAT1 (61 kD, Cat# YN3803, 1:1000, Immunoway), PPARα (52 kD, Cat# YT3835, 1:1000, Immunoway, Plano, TX, USA), and GAPDH (38 kD, Cat# YM3029, 1:1000, Immunoway, TX, USA).

Techniques:

Journal: Nutrients

Article Title: Lactiplantibacillus plantarum 06CC2 Enhanced the Expression of Intestinal Uric Acid Excretion Transporter in Mice

doi: 10.3390/nu16173042

Figure Lengend Snippet: Intestinal uric acid excretion transporter expression level. Effect of Lactiplantibacillus plantarum 06CC2 on uric acid excretion transporter expression levels in the intestine ABCG2 and PDZK1 transporter expression in the small intestine ( A ). ABCG2 and PDZK1 transporter expression in the large intestine ( B ). Data are the means ± SE, n = 8, * p < 0.05 vs. PO for the Tukey–Kramer test. Con: control diet, PO: potassium oxonate diet, PO + LP: potassium oxonate, 0.1% LP06CC2 diet, PO + L-LP: potassium oxonate, and 0.02% (low) LP06CC2 diet, SE: standard error.

Article Snippet: Primary antibodies ABCG2 (Anti-BCRP, ABCG2 antibody), PDZ domain-containing protein 1 (PDZK1, Anti-PDZK1), organic anion transporter 1 (OAT1, OAT1 Polyclonal antibody) and urate transporter 1 (URAT1, URAT1 Polyclonal antibody) were purchased from Proteintech Group, Inc. (Rosemont, IL, USA). β-Actin (Monoclonal Anti-β-Actin, antibody) was purchased from SIGMA-ALDRICH.

Techniques: Expressing, Control

Journal: Nutrients

Article Title: Lactiplantibacillus plantarum 06CC2 Enhanced the Expression of Intestinal Uric Acid Excretion Transporter in Mice

doi: 10.3390/nu16173042

Figure Lengend Snippet: Uric acid excretion transporter expression level in kidney. Effect of Lactiplantibacillus plantarum 06CC2 on uric acid excretion transporter expression levels in the kidney Expression levels of ABCG2, URAT1 and OAT1 transporters in kidneys. Data are the means ± SE, n = 8. Con: control diet, PO: potassium oxonate diet, PO + LP: potassium oxonate, 0.1% LP06CC2 diet, PO + L-LP: potassium oxonate, and 0.02% (low) LP06CC2 diet, SE: standard error.

Article Snippet: Primary antibodies ABCG2 (Anti-BCRP, ABCG2 antibody), PDZ domain-containing protein 1 (PDZK1, Anti-PDZK1), organic anion transporter 1 (OAT1, OAT1 Polyclonal antibody) and urate transporter 1 (URAT1, URAT1 Polyclonal antibody) were purchased from Proteintech Group, Inc. (Rosemont, IL, USA). β-Actin (Monoclonal Anti-β-Actin, antibody) was purchased from SIGMA-ALDRICH.

Techniques: Expressing, Control